Elizaveta Savochkina
Pavlov Institute of Physiology, Russia
Title: Comparison of autoprobiotic and probiotic in restoring intestinal microbiota and digestive function after experimental dysbiosis in rats
Biography
Biography: Elizaveta Savochkina
Abstract
Statement of the Problem: The interplay between the intestinal microbiota and epithelium is an important determinant of host health and nutritional status. Although probiotic bacteria are widely used to prevent and treat the intestinal dysbiosis, their effects on the microbiota and the digestive system compared with indigenous (autoprobiotic) strains have not been investigated. This study aims to evaluate the condition of microbiota and the activity of intestinal enzymes after correction of experimental dysbiosis in rats using probiotic (Enterococcus faecium L3) and autoprobiotic (12 strains of E. faecium).
Methodology & Theoretical Orientation: The experimental dysbiosis in rats induced by administration of ampicillin and metronidazole. Then rats were getting probiotic, autoprobiotic or phosphate buffer (PBS, control-2). In control-1, after administration of water, rats obtained PBS. At the end of the experiment, samples of epithelium and chyme were analyzed biochemically. Fecal samples were studied bacteriologically and by real time PCR.
Findings: After using probiotic or autoprobiotic, dyspeptic symptoms and microbiota disorders disappeared faster than in control-2. Autoprobiotic, but not probiotic, promoted bifidogenic effect and increased populations of E. coli and Fecalobacterium sp. in feces. Aminopeptidase N activity decreased in the epithelium or tended to decrease in the chyme after use autoprobiotic. Tissue-nonspecific alkaline phosphatase activity, separated from the total activity by inhibiting levamisole, tended to decrease in the epithelium after use autoprobiotic, and Intestinal alkaline phosphatase – in the epithelium and the chyme after use autoprobiotic or probiotic. Maltase activity in the chyme tended to increase after use autoprobiotic, but tended to decrease after use probiotic.
Conclusion & Significance: Specific effects of probiotic and autoprobiotic enterococci on intestinal microbiota and digestion have been demonstrated. The results may be useful for development of a new therapeutic strategy based on autoprobiotic usage in the correction of intestinal dysbiosis. The work was supported by the Russian Science Foundation Number: 16-15-10085.